CropReporter from Phenovation


Whole plant imaging of Photosystem II at megapixel resolution






CropReporter measures fluorescence images at megapixel resolution providing the
0-, I- and P-level of the Kautsky induction curve of Photosystem II. Fluorescence
parameters are calculated per pixel and displayed as images. Multispectral images
are captured using the same optical layout as for the fluorescence images and an
optical filter wheel. This yields images of the Chl-index (correlates with the amount
of chlorophyll), Ant-index (correlates with the amount of anthocyanin), NDVI
(normalized difference vegetation index), NIR and color.

CropObserver is to be positioned between 1 to 4 meters above the crop. A computer-controlled mirror system creates a rectangular measuring area containing more than 1.000 measuring points. At each measuring point a short pulse of laser light induces chlorophyll fluorescence emissions. A fluorescence sensor captures this signal in return. The first measurement covers all locations of the rectangular measuring area. Only locations with sufficient chlorophyll fluorescence signal are stored in the memory. In subsequent measuring cycles only these stored locations are measured. Since plants grow and move over time, at midnight (12 pm) all locations of the rectangular measuring area are measured again and checked on fluorescence intensity. From the number of accepted measuring positions the projected plant area is calculated. At each laser spot the Fo and Fm is measured and from this data the efficiency of photosynthesis of Photosystem II is calculated. At the same time the light intensity from the PAR quantum sensor (Licor 400-700 nm), the global solar radiation from the pyranometer (Licor 400-1100 nm) and crop height is determined, using single-point optical laser triangulation. From PAR light and Fq’/Fm’, the relative ETR (Electron Transport Rate which correlates with CO2 uptake) is calculated. The CropObserver can be monitored remotely by a computer using the internet. It uploads its data to the platform. On this platform all data from the CropObserver can be monitored and compared to other climate data.

CropReporter from Phenovation


Measuring fluorescence and spectral images of whole plants using high resolution
camera imaging and modulated LED excitation

• Camera frame rate of 4 up to 100 images/s at 14 bit
• Time-lapse recording
• All LED light sources for red, far-red, white, green, blue and uv
• Built-in computer for timed imaging, light control and data storage
• Pixel-to-pixel information on fluorescence and multispectral

Captured Images

• 30 fluorescence images of 1 Mp in 1 second
• 4 fluorescence images of 6 Mp in 1 second
• In darkness: F0, FI, Fm
• In the light: Fs’, FI’ and Fm’
• Spectral images at 6 bands
• Imaged area from 20 x 20 cm up to 70 x 70 cm2 (depending on model)
• High Depth of Field (DOF)

Images of Plants on Multiple Parameters

• Fv/Fm=ϕPo=(Fm-F0)/Fm (maximum quantum yield of PSII photochemistry)
• ϕRo=(Fm-FI)/Fm (quantum yield of reduction of end acceptors of PSI)
• ϕPSII=ϕPt=Fq’/Fm’=(Fm’-Fs’)/Fm’ (effective quantum yield of PSII photochemistry)
• ETR (Electron Transport Rate)
• Chl-index, Ant-index, NIR, NDVI, color in red, green and blue
• Raw 16 bit format on all captured images for using open source or other software

CropReporter from Phenovation
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